FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_input_300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_input_300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35862169
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58100.029.69000844
TATGCCG56450.029.45762346
GTATGCC62800.027.46790945
ATGCCGT77800.021.19617547
ACGTCTG100650.020.6684515
TGCCGTC79900.020.3368648
GCACACG110550.019.12950111
AGTCACC110250.018.43089728
CGTCTGA117050.018.03780616
ACACGTC125500.017.09817513
CACACGT133600.015.98404512
GCCGTCT106650.015.49455249
TCTCGTA103100.015.35894441
CTGAACT141850.014.90838619
TCGTATG126200.014.59804443
AGCACAC150300.014.41457810
TCTGAAC149150.014.22498918
CTCCAGT150100.014.18128124
CTCGTAT118550.013.70671542
GAGCACA155800.013.6621869
Produced by FastQC (version 0.11.2)