FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_chip_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_chip_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30390771
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41900.051.62410411
ACGTCTG44350.048.92759715
TATGCCG31100.047.92250446
CACAGTG44850.047.8444933
GTATGCC32150.047.21585545
TGCCGTC28150.045.71364248
ACACGTC48050.045.15994613
CACACGT48400.045.11870612
ATGCCGT30550.045.0585447
CGTATGC33700.045.04435744
GTCACAC49100.044.05498529
CGTCTGA49800.043.5734316
TCGGAAG54900.039.911853
GAGCACA55000.039.5794039
AGCACAC55650.038.86888510
ACAGTGA59750.036.1443534
CAGTGAT59400.035.89257435
AGAGCAC61050.035.7138068
CTGAACT62650.034.96657619
TCTGAAC67800.032.3103518
Produced by FastQC (version 0.11.2)