FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_chip_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_chip_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28361437
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29900.053.65281744
GTATGCC30300.053.2859245
TATGCCG30000.053.24360746
GCACACG45900.052.4617511
ATGCCGT29200.051.7491947
CACACGT47000.051.23383312
TGCCGTC28300.051.20061548
ACACGTC48500.049.79145413
ACGTCTG49550.048.8761915
CACTTAG50050.048.38859631
TAGGCAT49100.048.3411535
CGTCTGA51400.047.04998816
GAGCACA55550.043.534779
AGTCACT55400.043.52948828
CATCTCG38050.043.06689539
AGCACAC56450.042.84053410
CTGAACT58000.041.87454219
TCTCGTA37300.041.8055841
TCTGAAC58550.041.65758518
GCATCTC41250.040.06062738
Produced by FastQC (version 0.11.2)