FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_chip_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_chip_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31580456
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57800.051.3888411
ATGCCGT56500.050.6192247
TGCCGTC57250.049.775348
TATGCCG58400.049.50433346
CGTATGC61000.047.56443844
GTATGCC61150.047.1654545
ACGTCTG63300.046.87009415
CACACGT65800.045.5605312
ACACGTC67100.044.5236713
CGTCTGA75400.039.53164316
GAGCACA81500.036.784549
TCGGAAG85900.034.5460053
AGCACAC90400.033.3913910
AGAGCAC90850.033.1132858
TCTCGTA89500.031.76266541
AGTCACT94050.031.58347328
TCTGAAC99150.030.27101118
ATCTCGT94650.029.96143340
CTGAACT100500.029.8300619
AATCTCG95650.029.64819339
Produced by FastQC (version 0.11.2)