FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_chip_300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_chip_300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33791678
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG83450.053.36974311
TATGCCG83400.050.9633446
ATGCCGT82950.050.94860547
TGCCGTC83100.050.191848
CACGCCA88250.049.9999631
ACGTCTG89350.049.9230515
TCACGCC88500.049.8591630
CGTATGC86650.049.17143644
GTATGCC89350.047.84000845
CACACGT95750.046.83808512
ACACGTC100850.044.50381513
CGTCTGA105550.042.35882616
TCGGAAG119650.037.6066743
GAGCACA119700.037.4381839
AGCACAC122850.036.73047610
GCCAATA125000.035.4103234
AGAGCAC131350.034.3279958
TCTCGTA125950.034.2940541
CTGAACT138400.032.42969519
ATCTCGT137050.032.04488840
Produced by FastQC (version 0.11.2)