FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_chip_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_chip_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16935880
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAT230840.13630233563298746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG64250.049.07627515
CGTATGC43100.048.8277244
GTATGCC44750.048.6464945
GCACACG66100.047.4939711
TATGCCG45550.047.3379446
AGTCACC68250.045.090528
ACCGATG68850.044.74581532
ACACGTC70350.044.67365613
CACACGT70800.044.38958412
CGTCTGA73200.042.88756616
ATGCCGT48550.042.28089547
GTCACCG74450.041.42755529
CTGAACT81450.038.67058619
TGCCGTC51100.038.61770648
TCTGAAC81450.038.58553318
GAGCACA81950.038.5610549
AGCACAC82550.038.15540310
AGAGCAC89150.035.524578
TCGGAAG89550.035.1034663
TCTCGTA57850.034.4693641
Produced by FastQC (version 0.11.2)