FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_chip_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_chip_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19518585
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT61600.011.7047712
CCTGCGT63100.011.31767566
AACGCGG65700.011.02699
GCGTTAT66700.010.8101969
TCCTGCG66750.010.80211965
TCCTGGC69300.010.554069528
GTATGCC23400.010.46793345
CCTGGCC70000.010.25120329
CGTATGC22600.010.2278644
CGGCCTT71400.010.14654913
TATGCCG23750.09.87785446
GCTGGCC74800.09.87012940
ACGCCAG75450.09.7882571
ACGCGGC80750.09.56982310
TGCGTTA77350.09.3217868
ACGTCTG28800.09.10373315
GCACACG29250.08.72776711
CTGCGTT84450.08.70149667
GCAACGC87900.08.6345117
TTACGGT89750.08.30288121
Produced by FastQC (version 0.11.2)