FastQCFastQC Report
Fri 12 Feb 2021
HVG3CDRXX_n01_D487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG3CDRXX_n01_D487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20122742
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC547350.2720056739782282No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA464040.23060475555468535No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC401800.19967457715255704No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTAT340860.16939043396769685TruSeq Adapter, Index 7 (97% over 36bp)
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC302190.1501733710048064No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207680.10320661070941524No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT204920.10183502824813835No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)