FastQCFastQC Report
Fri 12 Feb 2021
HVG3CDRXX_n01_D328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG3CDRXX_n01_D328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30941062
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGTT7499972.423953644512913TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGGT4612901.4908667323700784TruSeq Adapter, Index 19 (97% over 37bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1501860.4853938109816657No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1371750.4433428949529916No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1326310.4286569090614925No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGAT1292830.4178363367100974TruSeq Adapter, Index 19 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT889530.2874917480208016No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC749970.242386638183266No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGTT671220.21693502310942012TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGGT627920.20294067475770547TruSeq Adapter, Index 19 (97% over 37bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT610320.19725244078564594No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTTT512420.16561163931606485TruSeq Adapter, Index 19 (97% over 37bp)
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT505180.16327170670483127No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA487780.1576481117551815No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC395370.12778165145074852No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA386220.12482441617550166No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA381340.12324722402870333No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG373750.12079417312825269No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG360690.11657324496489488No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC360670.1165667810626539No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC344510.11134394805194468No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC330250.10673518575412828No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTGT322710.10429829460928004TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)