FastQCFastQC Report
Fri 12 Feb 2021
HVG3CDRXX_n01_D316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG3CDRXX_n01_D316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT994.600371747211896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTAT431.9981412639405203TruSeq Adapter, Index 10 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA321.486988847583643No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGGAT140.6505576208178439TruSeq Adapter, Index 10 (97% over 37bp)
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC90.4182156133828996No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC90.4182156133828996No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTTT80.37174721189591076TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGTAT80.37174721189591076TruSeq Adapter, Index 10 (97% over 37bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT70.3252788104089219No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC70.3252788104089219No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT60.2788104089219331No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTGT60.2788104089219331TruSeq Adapter, Index 10 (97% over 37bp)
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT50.23234200743494424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGGTT50.23234200743494424TruSeq Adapter, Index 10 (97% over 37bp)
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG50.23234200743494424No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG50.23234200743494424No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC50.23234200743494424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGAT50.23234200743494424TruSeq Adapter, Index 10 (97% over 37bp)
GCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC40.18587360594795538No Hit
GCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCAC40.18587360594795538No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC40.18587360594795538No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG40.18587360594795538No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA40.18587360594795538No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC40.18587360594795538No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGTT30.13940520446096655TruSeq Adapter, Index 10 (97% over 37bp)
CTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCA30.13940520446096655No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA30.13940520446096655No Hit
TCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGA30.13940520446096655No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA30.13940520446096655No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG30.13940520446096655No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA30.13940520446096655No Hit
AGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTG30.13940520446096655No Hit
CACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAA30.13940520446096655No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA30.13940520446096655No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC30.13940520446096655No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT30.13940520446096655No Hit
GTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGC30.13940520446096655No Hit
CTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGA30.13940520446096655No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA30.13940520446096655No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA30.13940520446096655No Hit
CCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGC30.13940520446096655No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC30.13940520446096655No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT30.13940520446096655No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA30.13940520446096655No Hit
GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC30.13940520446096655No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA30.13940520446096655No Hit
GTGTGAGAATCAAGCCTGCTCAATGCTCATCATGGATCCCTGTGAATTAC30.13940520446096655No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA30.13940520446096655No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG30.13940520446096655No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT30.13940520446096655No Hit
AAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGC30.13940520446096655No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC30.13940520446096655No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT30.13940520446096655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)