FastQCFastQC Report
Fri 12 Feb 2021
HVG3CDRXX_n01_D161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG3CDRXX_n01_D161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12119741
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTCGTAT534020.44061997694505184TruSeq Adapter, Index 3 (97% over 36bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC516830.4264365055325852No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC419910.3464677999307081No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA377420.311409294967607No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC254940.21035102977860665No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220420.18186857293402559No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT202260.16688475438542788No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA161630.13336093568336155No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC159950.13197476744758818No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA157880.13026681015708175No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCGCGTAT136830.11289845220289774TruSeq Adapter, Index 3 (97% over 36bp)
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA127960.10557981395807056No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC125600.10363257762686512No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC121200.10000213700936347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)