FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatreptsu-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatreptsu-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13733378
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC201080.14641699951752585TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151080.11000935094046055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30150.045.50662246
CGTATGC35350.039.1116144
GTATGCC36250.038.0431745
CTAATCG39000.035.00751533
TCGTATG41000.034.1490743
ATGCCGT43050.032.03309647
ACGTCTG46700.030.27494815
TGCCGTC46700.029.37890248
CTCGTAT47650.028.72285842
GCACACG50650.028.25939211
ACTAATC49950.027.7541132
GCCGTCT50650.027.5705649
CACACGT53400.026.67301412
CACTAAT55500.024.9788831
ACACGTC58100.024.39474113
AGTCACT62800.022.18499828
CACGTCT64600.021.94024314
GTCACTA65100.021.29419529
CGTCTGA67900.020.71938316
TCTCGTA65700.020.67211541
Produced by FastQC (version 0.11.2)