FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatreptsu-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatreptsu-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8100541
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC397180.490312930951155TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47400.059.88210746
CGTATGC50050.056.8555944
GTATGCC51200.055.64692745
ATGCCGT53050.053.57044247
TCGTATG54650.052.26208543
ACGTCTG55500.051.76741815
GCACACG56450.051.33048211
CACGTAG56050.050.96865531
GTCACGT56050.050.96519529
TGCCGTC57250.049.45667348
AGTCACG58600.048.56670828
TCACGTA58800.048.5223830
CTCGTAT58500.048.40588442
CACACGT60150.048.2308812
GCCGTCT59500.047.82145349
ACACGTC62600.046.1196413
CACGTCT65100.044.4022914
ACGTAGA65600.043.92252732
CGTAGAG65750.043.66150333
AGCACAC68750.042.91052610
Produced by FastQC (version 0.11.2)