FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatreptsu-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatreptsu-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11462329
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC426910.37244612329658305TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54700.056.7557346
CGTATGC57650.054.0957544
GTATGCC57950.053.87610245
TCGTATG60950.050.53583543
ATGCCGT60550.050.5206447
ACGTCTG69750.048.9680415
GCACACG71700.047.53845211
TGCCGTC65600.046.7917448
CACACGT74150.046.06191612
GCCGTCT67350.044.3277249
ACACGTC76750.044.3191313
CTCGTAT69600.044.00639742
CGTCTGA83650.040.95659616
CACGTCT86000.039.8372614
AGCACAC90250.038.19375210
GAGCACA92650.037.2421579
TCTCGTA83600.036.63690641
TCGGAAG96550.035.5235943
ATCTCGT92700.033.56992740
ATCGGAA103900.033.011772
Produced by FastQC (version 0.11.2)