FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepstw-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepstw-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746807
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC425470.2885166938171768TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55250.057.711946
CGTATGC63700.050.0596344
GTATGCC63900.049.95705445
ATGCCGT66400.048.17912347
TCGTATG69400.046.3022743
TGCCGTC73850.043.460448
ACGTCTG78300.042.37054415
CTCGTAT74300.041.6010442
GCCGTCT82400.038.906449
ACTAATC79100.038.6805236
CACACGT87800.037.78577412
GCACACG87750.037.6077711
CCAACTA85950.037.4714733
AACTAAT88650.036.1720135
ACACGTC92050.035.964913
CAACTAA98050.033.0612534
CGTCTGA100400.033.0095516
CACGTCT101650.032.43104614
TCTCGTA96400.032.3178441
CTAATCT98400.031.16462737
Produced by FastQC (version 0.11.2)