FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepstw-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepstw-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338108
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC395920.38297142958847014TruSeq Adapter, Index 16 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103740.10034718151522504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49650.061.1165946
CCCGTCC53250.057.7245133
CACCCGT55550.055.59006531
CGTATGC54950.055.41798844
ACCCGTC58250.052.89109832
ATGCCGT57850.052.6345647
GTATGCC58500.052.35342445
GTCACCC60000.051.52324729
TCACCCG61200.050.57116730
TCGTATG59950.050.44760543
ACGTCTG63950.049.08635315
GCACACG65100.048.43402511
TGCCGTC62550.048.39936448
CACACGT67850.046.4707412
GCCGTCT65450.045.66487549
ACACGTC69950.044.9255413
CTCGTAT66200.044.1541842
CCGTCCA70250.043.75580234
CGTCTGA76000.041.39587416
CACGTCT77650.040.7862614
Produced by FastQC (version 0.11.2)