FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepstw-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepstw-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007051
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC543030.41748894503450473TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142930.10988655307033086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66500.059.5799446
CGTATGC72200.054.87837244
GTATGCC73950.053.768445
ATGCCGT74850.052.5593347
TCGTATG77000.050.82176643
GCACACG83900.049.84456311
ACGTCTG84000.049.78426715
TGCCGTC82950.047.42640748
CACACGT90300.046.46685412
ACACGTC91600.045.57781213
CTCGTAT84950.045.45038242
GCCGTCT86550.044.8048149
CGTCTGA101950.041.15621616
CACGTCT103550.040.68958314
AGCACAC108600.038.8944510
GAGCACA110550.038.429689
TCGGAAG113150.037.0543443
TCTCGTA104500.036.88089441
CATCTCG108050.035.9945239
CAGTCAC120550.035.05127727
Produced by FastQC (version 0.11.2)