FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepst-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepst-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695835
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC446380.35159562171373526TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55400.056.3557546
CGTATGC60750.051.2223144
GTATGCC61050.051.0842745
ATGCCGT64800.048.3425447
TCGTATG66200.047.26992843
TGCCGTC69250.045.48876648
CTCGTAT73000.042.4846142
ACGTCTG76550.041.6470715
GCCGTCT76700.041.29609349
GCACACG81600.039.32650411
CACACGT83950.038.35112
ACACGTC84300.037.776213
CGTCTGA92700.034.42956516
TCTCGTA91800.033.97404541
CACGTCT96150.033.23005714
AGCACAC99500.032.67386610
CAGTCAC103550.030.72971327
GATCTCG105050.029.65754939
GAGCACA111550.029.4264419
CTGAACT111150.029.25059319
Produced by FastQC (version 0.11.2)