FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepst-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepst-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12604307
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC2659.5934065E-613.2119671
CGCCCCT5255.456968E-1212.6709071
GCCGAAT16850.012.2592951
CGGGCCT8000.012.2541011
GCCGGTT31150.011.2397161
CCCCCCT4301.0640379E-610.5849371
TATGCCG11350.010.48459648
GGCGAAT31050.010.3738421
GCGGGCA9500.010.31924251
GCCGGAT23800.010.0033471
CTCGGTT45350.09.9592311
GCGGGCT7554.0017767E-119.73835851
GCGGGGT5401.8869287E-79.7254761
GGGGAAT28100.09.3447651
GCCCGCC4158.83991E-59.2802131
GCGGGTT30050.09.2044111
GGGGACT12600.09.1697351
GCCCGAT9550.09.1653711
GGCAGCT55150.09.0782881
GTCGAAT60800.09.0408541
Produced by FastQC (version 0.11.2)