FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepst-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepst-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8111200
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC231870.2858639905316106TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82870.10216737350823553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31550.053.3615646
GTCACGC32100.053.108729
GTATGCC34000.049.6202645
CGTATGC34050.049.341844
TCACGCC35650.047.82047330
ATGCCGT36450.046.6679747
TCGTATG36850.045.68817543
CACGCCA37400.045.21127331
TGCCGTC39400.043.1732648
ACGTCTG39650.042.98296415
TATCTCG40150.042.02249539
GCACACG41250.041.48592811
CTCGTAT40700.041.1095942
GCCGTCT42300.040.12941449
ACGCCAA42600.039.77421632
CACACGT43150.039.65920312
ACACGTC43450.039.3851313
CGCCAAT44600.038.14713333
AGTCACG45800.037.37418728
CGTCTGA51400.033.4294316
Produced by FastQC (version 0.11.2)