FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepshahdara_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepshahdara_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12001040
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT647660.5396698952757428TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149310.12441421743448901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG78750.062.75527248
GTATGCC86750.057.1709647
CGTATGC86700.057.08329846
ATGCCGT88000.056.197249
TCGTATG92800.053.3323945
TGCCGTC93600.052.9460550
TATCTCG93750.052.53498541
CTCGTAT95850.051.4529544
ACGTCTG101050.049.69585415
CACACTG101100.049.31126831
GCCGTCT100900.048.97651751
GCACACG106450.047.47055411
ACACGTC108300.046.530413
CACACGT109300.046.04065312
TCTCGTA110250.044.82799543
CACGTCT114050.044.36878214
TGATATA112400.044.35233736
ACACTGA113700.043.72334732
CGTCTGA115700.043.52414316
ACTGATA115100.043.25055734
Produced by FastQC (version 0.11.2)