FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepshahdara_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepshahdara_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9806971
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106690.1087899617527165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13250.039.886748
CGTATGC18250.029.91880646
GTATGCC18850.028.59438947
ATGCCGT20650.025.76199549
ACGTCTG24250.022.51196915
GCACACG24650.022.00492111
TCGTATG25600.020.91973345
TCCGTAT26050.020.83038537
TATCTCG26850.019.94734241
TGCCGTC27700.019.58379450
CGTATCT28450.019.07199939
GTTCCGT28800.018.72000735
CACACGT29150.018.48769612
CTCGTAT28400.018.36403744
GCCGTCT31400.017.16451551
CCGTATC31950.016.98316838
ACACGTC32050.016.8146913
AGTTCCG34200.016.58330534
TTCCGTA34950.015.72627336
CGTCTGA37100.015.09215116
Produced by FastQC (version 0.11.2)