FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepshahdara_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepshahdara_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10325953
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC260740.2525093809743275TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114280.11067259360951961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36450.055.59678346
CGTATGC39750.051.0714844
GTATGCC41500.049.3383745
ATGCCGT43750.046.3198447
TCGTATG45500.044.46461543
ACGTCTG48500.043.51004415
GCACACG50350.041.98127711
TGCCGTC48200.041.970448
CACACGT51850.041.23903712
ACACGTC55850.038.2228213
GCCGTCT53500.037.8114949
CTCGTAT47900.037.1228242
CACACAG61250.034.4100431
CGTCTGA62950.033.52259416
CACGTCT64150.033.3317814
CACAGTG63000.033.23111733
GAGCACA67850.032.442859
ACACAGT68500.030.61403532
AGCACAC70450.030.35137710
TCACACA73100.028.97464430
Produced by FastQC (version 0.11.2)