FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepoy-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepoy-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14742449
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1123740.7622478463381491TruSeq Adapter, Index 22 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157080.10654946135475864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG125000.063.78425646
CGTATGC132550.060.2078244
GTATGCC134650.059.37199845
ATGCCGT137400.057.87446247
TCGTATG139450.057.15498443
TGCCGTC143850.055.23042748
CTCGTAT143200.055.074642
ACGGTAG149350.053.91343732
ACGTCTG153950.052.96423315
GCCGTCT151750.052.21466449
GTCACGG156750.051.45775229
AGTCACG157700.051.12400428
GGTAGCA161650.049.80979534
GCACACG164250.049.74941311
ACACGTC166400.049.10661713
CACACGT166700.049.08122612
CGGTAGC169750.047.5163133
CACGTCT173350.047.1576914
TCTCGTA166950.047.051441
CGTCTGA175900.046.59373516
Produced by FastQC (version 0.11.2)