FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepoy-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepoy-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8003502
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT131280.16402819665691343TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84320.10535388133844409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17500.051.8004348
GTATGCC23050.039.0244347
CGTATGC23550.038.4931346
ATGCCGT25650.035.2040949
TGCCGTC26350.034.66562350
TCGTATG26900.034.22154245
ACGTCTG27900.033.23860515
GCACACG29750.031.17166511
GCCGTCT30100.030.81170751
CTCGTAT30900.029.33901444
CACACGT32650.028.72451612
ACACGTC33500.028.20460913
CACGTCT37250.025.36537614
AGCACAC40350.024.28374110
CGTCTGA39700.023.88785616
ACAGTCA39350.023.57808332
CACAGTC40050.023.34081831
GAGCACA45150.022.2444939
TCTCGTA40750.022.16135443
GTCACAG46150.020.5574729
Produced by FastQC (version 0.11.2)