FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepoy-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepoy-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6545295
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC186600.28509028240896706TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73060.11162216523472204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23400.057.13839746
CGTATGC26400.050.7799244
GTATGCC26300.050.70565845
ATGCCGT28600.046.8712747
TCGTATG29750.045.29784843
ACGTCTG30800.044.87977215
GCACACG31450.043.840611
ACTGACC32750.041.5873832
CTCGTAT31450.041.2946342
TGCCGTC32650.040.84282348
CACACGT34650.040.0948212
GCCGTCT33400.039.81876849
CACTGAC35400.038.57428731
ACACGTC37750.036.70955713
CGTCTGA41600.033.7327716
AGCACAC42050.033.62092610
CACGTCT41300.033.55439414
AGTCACT41150.033.43460528
TCTCGTA43550.030.46524241
GAGCACA46700.030.3481669
Produced by FastQC (version 0.11.2)