FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepn13_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepn13_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14670634
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT2048821.396544961860544TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182530.12441861749124135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG227450.065.12170448
CGTACGT228150.065.0014334
CGTATGC229850.064.4582746
GTACGTA231500.064.2124735
CCGTACG232650.063.91011433
GTATGCC232950.063.67517547
TACGTAA234850.063.2368936
ATGCCGT237450.062.5086249
TCGTATG238750.061.92749845
TGCCGTC242100.061.20641350
ACGTAAT244700.060.5763137
CTCGTAT242500.060.1479344
ACCGTAC247300.060.08244332
GCCGTCT252450.058.5439651
ACGTCTG271150.055.5610415
TCTCGTA263250.055.2745343
CGTAATC267300.054.61490638
CACCGTA281400.053.00126331
AATCTCG276850.052.6753441
CGTCTGA287250.052.5200116
Produced by FastQC (version 0.11.2)