FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepn13_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepn13_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11038568
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122040.1105578187315601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12050.024.10846
CGTATGC16300.018.25269744
GTATGCC17750.016.95867345
ATGCCGT19200.015.31243647
GCGGGGT4950.014.8531321
TCGTATG21900.013.10597743
CGGGCCT7950.012.7712921
TGCCGTC23450.012.53720448
ACGTCTG25300.012.31069615
CGCGCAT5401.1823431E-1011.6703191
GCACACG26150.011.50890411
CGGGCAT16150.011.4896631
GGGCGCT3054.0590086E-511.4790021
GCGGGAT18750.011.3902311
GGCGTAT12800.011.2144471
GCGGTAT13400.010.9735831
GTCGGAT33600.010.7325251
CTCGTAT28100.010.713057542
CGGGGAT19200.010.5762261
GCCGAAT16750.010.4510321
Produced by FastQC (version 0.11.2)