FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepn13_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepn13_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5862651
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC333900.5695375692668726TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37550.061.70653546
CGTATGC39650.058.0872344
GTATGCC40550.057.40222545
ATGCCGT41850.055.28174247
TCGTATG42100.054.29161543
GCACACG45700.051.84153411
ACGTCTG46050.051.59993715
TGCCGTC45300.051.302948
CACACGT46950.050.6103712
CTCGTAT45100.049.98297542
ACTTAGG47800.049.29088632
CACTTAG48550.048.6015531
GCCGTCT47400.048.36129449
TTAGGCA49700.048.1805534
ACACGTC49850.047.9469413
TCACTTA50150.047.11955330
TAGGCAT51400.045.77010735
CACGTCT53750.044.3381614
AGTCACT53700.044.06600628
CGTCTGA53600.044.00521516
Produced by FastQC (version 0.11.2)