FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepmt-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepmt-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895503
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT476450.4814813355116966TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57400.059.99758548
CGTATGC60900.056.9528846
GTATGCC61750.055.99859647
CGTGGCC62850.055.03436733
ATGCCGT64600.053.4711949
ACGTGGC66350.052.3977432
TCGTATG67550.051.4526145
GCCTTAT67950.051.10983737
TGCCGTC68100.050.5683550
CACGTGG69100.050.26177631
TATCTCG69100.050.0522841
GTCACGT71350.048.77024529
TCACGTG71600.048.65057430
GCCGTCT72350.047.64622551
CTCGTAT72500.047.60193644
GTGGCCT75500.046.04499434
GGCCTTA77100.045.2715436
ACGTCTG78800.044.98707215
AGTCACG78250.044.55808628
ACACGTC88000.040.4831213
Produced by FastQC (version 0.11.2)