FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepmt-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepmt-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8306115
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8650.023.87258746
CGTATGC12150.016.42047344
GTATGCC13200.014.84886845
GCCGGAT17950.013.2642671
ATGCCGT16200.012.9627547
CGCCCTT11600.012.6773971
CGGGGCT10450.012.3972181
ACGTCTG17900.012.31697415
GCGGGCT5751.8189894E-1212.17869951
GGCGAAT21300.012.0000211
TCGTATG17900.011.53681843
CGCGCTA3054.0554063E-511.4799221
GGGCGCT3054.0554063E-511.4799221
CGGGCCT6006.366463E-1111.0876911
TATCTCG19800.010.78408139
TGCCGTC19050.010.6559348
GGGGAAT17700.010.4843461
CTCGTAT20850.010.07274842
GCGGGGT2800.002947548610.0039321
CCCCGCT6704.7293724E-109.92927551
Produced by FastQC (version 0.11.2)