FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepmt-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepmt-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670367
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC383790.5753656432996865TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71240.10680072026021956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44950.061.4366946
GTATGCC46950.058.89810645
CGTATGC48200.057.37152544
ATGCCGT49850.055.25652747
ACGTCTG52900.054.51175715
TCGTATG50450.054.11940843
TATCTCG51350.053.03811339
GCACACG55450.052.25774811
CCGATGT54700.051.6473633
TGCCGTC53300.051.4168348
GCCGTCT53650.050.62207449
CACACGT57400.050.54265612
CTCGTAT53800.050.49077642
ACACGTC58750.049.5599513
GATGTAT60550.046.7149535
ACCGATG62600.045.13023832
CGATGTA63000.045.06495734
CGTCTGA64500.044.9790416
CACGTCT64750.044.64323414
AGCACAC66050.044.34771710
Produced by FastQC (version 0.11.2)