FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepmr-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepmr-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17848550
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT9385765.258556017155455TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197750.11079331374257292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC1015400.067.8753133
TATGCCG1017950.067.69585448
GTCCGCA1021300.067.42760534
CGTATGC1025550.067.3392146
GTATGCC1025650.067.2983347
CCGCACA1020100.067.23543536
ACGTCCG1026600.067.22517432
TCCGCAC1025400.067.1299335
ATGCCGT1026900.067.06212649
TCGTATG1030100.066.9762645
CGCACAT1021500.066.89921637
TGCCGTC1033450.066.6086950
CTCGTAT1027350.066.3997444
GTCACGT1045700.066.1247129
AGTCACG1047150.066.0745628
ACGTCTG1055550.066.0691415
GCCGTCT1042600.065.9495351
TCACGTC1052450.065.6749130
GCACACG1075250.064.98324611
GCACATC1041300.064.9488738
Produced by FastQC (version 0.11.2)