FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepmr-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepmr-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742749
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC299190.38641314602862625TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82860.1070162548211236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36250.061.12066746
GTATGCC40350.054.91084345
CGTATGC41350.054.0064944
ATGCCGT41100.053.651647
TCGTATG44200.050.2875743
ACGTCTG46400.048.2701515
TGCCGTC47100.046.889848
GCACACG47950.046.63651311
ACACGTC50550.044.6531813
CACACGT50200.044.4762312
AGTCACG50300.044.26152428
GCCGTCT50750.043.51546549
CTCGTAT49800.043.43925542
GTCACGA51550.043.18909529
CGATCAG52500.042.14304433
AGCACAC56550.039.91544310
CACGTCT59100.038.19369514
CGTCTGA58650.038.1881516
TCACGAT58550.038.02607330
TCTCGTA58200.037.1699141
Produced by FastQC (version 0.11.2)