FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_nutrinetatrepmr-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_nutrinetatrepmr-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9482815
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC441340.46541032383316555TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97740.10307065992534917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52150.062.6839446
CGTATGC58250.056.66515444
GTATGCC58500.056.06340845
ATGCCGT60600.054.05972347
TCGTATG61900.053.32496643
ACGTCTG63600.051.8883915
TGCCGTC67450.048.8287948
CTCGTAT66200.048.64666442
GCCGTCT69200.047.54237449
GCACACG69650.047.38146211
ACACGTC70400.046.72731413
CACACGT72900.045.36489512
CACGTCT75800.043.5831314
TCACGAT76200.042.9564135
AGCACAC77700.042.38204610
CGTCTGA82250.040.42086816
TCGGAAG81900.040.337653
TCTCGTA80150.040.18020241
GAGCACA85800.039.0739179
GATCTCG83750.038.2456839
Produced by FastQC (version 0.11.2)