FastQCFastQC Report
Wed 29 Nov 2017
HVFKYAFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFKYAFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16809767
Sequences flagged as poor quality0
Sequence length50
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAG5485700.043.952428
TCCCCGA4393300.043.9417237
GCCCCGA1270400.043.895497
CCCGAGG5515300.043.878749
GGTCTCA2932050.043.80086514
ACGAGGT4912400.043.7755410
TCCACGA3932300.043.7695057
TGTCCCC1222800.043.751935
GTCCCCG4417350.043.715776
GTCTCGA1840600.043.70435715
CCACGAG4415000.043.6983648
CACGAGG4582900.043.686899
GTCTCAG2631200.043.6565615
AGTCCCC1157750.043.637495
GGTCTCC1210100.043.60144414
CAATGGG265500.043.5686951
GTCTCCT1101350.043.55481315
CCCCGAT129650.043.554348
GTCTCTA1598100.043.5344715
GGCCCCG1278850.043.5215266
Produced by FastQC (version 0.11.2)