FastQCFastQC Report
Thu 1 Aug 2019
HVFH5BGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFH5BGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537764044
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7956860.14796191914980467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG18001850.061.8811042
AAGCAGT18170000.061.519561
GCAGTGG20095150.055.356333
AGTGGTA21695050.051.3289455
CAGTGGT22985700.048.4026534
GTGGTAT24205400.045.977396
GGTATCA25052650.044.3521588
TGGTATC25510400.043.5943957
GTATCAA25532750.043.3946089
ACGCAGA24288700.025.5936314-15
CAACGCA24524100.025.34661512-13
GCAGAGT25323700.024.6706616-17
AGTACAT25215900.024.63514120-21
TACATGG25232800.024.482622-23
AGAGTAC25756100.024.29274618-19
ATCAACG26016000.024.03124610-11
CATGGGG15735850.023.75652324-25
GTACATG24345700.022.76912922-23
CGCAGAG24886450.022.45683516-17
TCAACGC25002750.022.23708512-13
Produced by FastQC (version 0.11.5)