FastQCFastQC Report
Thu 1 Aug 2019
HVFH5BGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFH5BGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537764044
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAGC467312088.689909361065427No Hit
GCTACCTG463854768.625618710945277No Hit
CTGTAACT458001558.516775249480979No Hit
TGCTGTAA451606658.397858782838222No Hit
TCTAGCGA430894368.01270305829521No Hit
CTGGACTC420018827.810466777879259No Hit
GACCCTAC410226827.628379483102816No Hit
AAAGTGCT408858877.602941746696623No Hit
TGTAGAAG401313557.4626326262899045No Hit
GCCTTGGT381371647.091802515528539No Hit
AAACCTCA347477646.461526088940227No Hit
AGAGTGTG315834485.873105194069093No Hit
GGGGGGGG179803313.3435353666003005No Hit
GCCCCTAC14783040.2748982600257298No Hit
CTGGCCTC5961790.11086256261491519No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)