FastQCFastQC Report
Thu 1 Aug 2019
HVFH5BGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFH5BGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537764044
Sequences flagged as poor quality0
Sequence length29
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAA2873600.020.5344621
AACGAAG2347900.020.3563692
AATGCGT3709550.020.2919373
GCACGCA2834900.020.2390123
CGGCCCA2064200.020.2216059
GGCGCAA1892800.020.0713924
GACCGTG2212300.020.0237051
CGCAGCT2716600.019.9627236
GCGCAAC1897900.019.9543485
GGCCCAA2091150.019.92581710
ACGCAGC2649050.019.8739265
CGTCGGC2136350.019.761596
CCGATGG2560750.019.7455841
ACCGTGA2028400.019.6820432
GAATGCG2566400.019.6441612
GCATCGA2935650.019.6348577
AGCGACC1434600.019.5353479
GCAGCTC2725250.019.462137
ATCGAGC2543400.019.4344889
ACGAAGT2468400.019.3590013
Produced by FastQC (version 0.11.5)