FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85225265
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10197311.1965125599785462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT71650.022.07684
TCATTAA928350.018.33963894-95
TATCATT1302800.017.5261294-95
GTATCAT1503700.017.04531594-95
CGTATCA1753400.016.09852894-95
CCGTATC2026700.015.17813494-95
GTACCGT63750.014.4555456
GTCTATG124600.014.0285021
ATCATTA1508800.013.99033394-95
AGTACCG88100.013.85703855
GCCGTAT2478750.013.77941394-95
GTATAGG145500.013.7436051
CGCCGTA2944600.013.22965594-95
TACCGTG74850.012.9443617
GCCCTAG71950.012.8731671
CACTGAG135950.012.7513437
ACTGTCC73150.012.5954398
ACTGTGC88350.012.5786848
TAGACGG53550.012.4188615
TACACCG88650.012.2171335
Produced by FastQC (version 0.11.5)