FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_SAL60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_SAL60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71189877
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG79700.025.688875
TATACCG239650.020.9122645
GTACCGT120400.020.3586626
GTCTAGG212600.020.0882851
GGACTGC163750.018.682316
AGTACCG175000.018.4585195
TAGAGCG196800.018.3690015
GACGGTC99900.018.1630367
CCGTATC2496900.017.87853494-95
CCTAGAC220100.017.848853
GTATCAT1761750.017.70060394-95
CGTATCA2069500.017.53579994-95
GTCTATG256150.017.3591291
CTAGACA256650.017.3059864
ATCATTA1335750.016.71005694-95
CGCCGTA4074750.016.67979694-95
TATCATT1603800.016.65980794-95
TACCGTG148900.016.58827
GCCGTAT3370850.016.56318794-95
GTGTATG309050.016.462931
Produced by FastQC (version 0.11.5)