FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_SAL5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_SAL5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97775738
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG92450.024.817825
TATACCG266300.022.3334875
TCATTAA2949400.022.31695694-95
ATCATTA3770250.021.96286294-95
GTACCGT144550.021.196546
GTCTAGG269000.021.1742611
GGACTGC189700.020.7842396
CATTAAA2100200.020.7162294-95
TATCATT4666450.019.7221694-95
AGTACCG209000.019.5013375
CCTAGAC276550.019.221143
TAGAGCG218750.018.6538495
CTAGACA283900.018.5562214
TACCGTG187150.018.357
GTATCAT5459850.017.88456794-95
ACTGTCC142900.017.849648
TACACCG197800.017.6516155
GTATAAG459900.017.5910851
GTCTATG303700.017.3784331
GCCCTAG149550.017.37571
Produced by FastQC (version 0.11.5)