FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_SAL5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_SAL5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50701749
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG926250.18268600556560682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA1167350.016.34337694-95
GCCGTAT3026850.016.149694-95
CGCCGTA3698700.015.54379294-95
CCGTATC2393550.015.37099694-95
TCATTAA1331250.014.447185594-95
CGTATCA1967550.013.78978594-95
ATCATTA1456200.012.77848192-93
TATACCG142200.012.29345
GTATAGG242500.011.9496731
TCGCCGT4538300.011.90617894-95
TATCATT1612100.011.45285592-93
ATTAAAA1346000.011.30687394-95
TGGTCGC5252150.011.17365790-91
GTATCAT1739700.011.11258694-95
CTATACT281850.011.0225864
GTCGCCG5489750.010.80343892-93
CCTATAC256500.010.7785053
GTCTAGG135450.010.7670641
GGTCGCC5282850.010.45509592-93
ACTGAGC200100.010.2310988
Produced by FastQC (version 0.11.5)