FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_SAL180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_SAL180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42964952
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG455410.1059956962130436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC804800.016.6440594-95
CGCCGTA1407600.016.32617894-95
GCCGTAT1142650.016.07321494-95
CGTATCA679450.014.688127594-95
TCGCCGT1776200.012.86994894-95
TATACCG139100.012.8404765
TAGACGG69800.012.1139285
CCTAGAC143750.012.0615863
TGGTCGC2020350.011.46490490-91
GGTCGCC2049100.011.39217892-93
GTATCAT644050.011.26942394-95
GTCTAGG141950.011.2445081
CTAGACA203600.011.1057944
TAGAGCG148100.010.9375415
GTGGTCG2177900.010.67696890-91
GTCGCCG2219600.010.63264192-93
TAGAGGT189850.010.5339654
TAAGGTG208400.010.48529155
TCATTAA537150.010.45691394-95
TAGACTG114700.010.43656355
Produced by FastQC (version 0.11.5)