FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_SAL180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_SAL180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82547904
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3190350.024.21281294-95
ATCATTA4218800.023.14833894-95
CATTAAA2233000.022.5812594-95
TATCATT5324050.020.60006794-95
TATACCG219050.020.5150595
GTACCGT122150.019.7947146
GTCTAGG217100.019.6498181
TAGACGG82400.019.4279775
AGTACCG164000.018.943445
GTATCAT6387650.018.32668594-95
CCTAGAC216350.017.1921423
TACCGTG151950.016.9428377
CTAGACA231700.016.8937514
GGACTGC162850.016.2477176
GTCTATG240500.016.2169841
GACTGTG95500.016.1646967
GTATAAG350800.015.9795471
CGTATCA7625650.015.87682594-95
TAGAGCG187300.015.6231225
ACTGTCC126700.015.5582178
Produced by FastQC (version 0.11.5)