FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_SAL15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_SAL15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67980617
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT98000.022.0068046
ATCATTA2430100.021.74175894-95
TAGACGG62900.021.6749675
TCATTAA1913100.021.58633294-95
GTCTAGG190250.021.1744981
TATACCG182500.020.7974995
TATCATT3023050.019.75168294-95
AGTACCG143450.019.6041665
GGACTGC135450.019.3942666
CATTAAA1378500.019.30854294-95
CCTAGAC190550.018.5477223
GTATCAT3580700.018.5042794-95
TACCGTG123650.017.9780487
CTAGACA196550.017.5223434
TACACCG130800.017.3235875
GTGTATG238500.017.1696241
GTCTATG212000.017.0750121
GTATAAG315700.016.5222661
CGTATCA4287700.016.47119194-95
TATACTG308700.016.3115965
Produced by FastQC (version 0.11.5)