FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101737024
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG104700.027.3134845
TCATTAA3015300.022.90276394-95
ATCATTA3944500.022.12879894-95
CATTAAA2125900.021.32375394-95
GTCTAGG271450.020.5108491
TATACCG298750.020.4325475
TATCATT4923850.020.02966194-95
GTACCGT167900.019.6918496
ACTGTCC164250.018.2768158
GTATCAT5876900.018.27392694-95
AGTACCG242350.017.6019445
CCTAGAC276950.017.358213
GGACTGC211450.017.2986016
CTAGACA312400.017.0915974
GTGTATG365750.016.8072191
TAGAGCG257900.016.7985125
TACCGTG218100.016.6389927
GACGGTC136300.016.309457
TACACCG201500.016.2431965
CGTATCA7034100.016.1995394-95
Produced by FastQC (version 0.11.5)