FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108287194
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3752350.024.1664394-95
ATCATTA5064750.023.6149894-95
TAGACGG99500.023.0117245
CATTAAA2621300.022.36401494-95
TATCATT6469800.021.6567394-95
GTACCGT161650.020.9820486
TATACCG277200.020.3928975
TAGAGCG253750.019.8250875
GTCTAGG268900.019.7512341
GTATCAT7925500.019.45870894-95
ACTGTCC150450.018.8166358
AGTACCG235000.018.2332425
TACCGTG208050.017.9221557
CCTAGAC274400.017.5887263
TACACCG193450.017.1891545
CGTATCA9658650.016.9410394-95
GTGTATG335200.016.7657891
GGACTGC190950.016.6927646
GTATAAG447150.016.3929061
AAGACCG232550.016.3826145
Produced by FastQC (version 0.11.5)