FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55276869
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC1699400.016.27879794-95
CGTATCA1440350.015.94666794-95
GCCGTAT2249400.015.13869694-95
CGCCGTA2652100.014.63284394-95
GTATCAT1273250.014.53642394-95
GTCTAGG156150.012.9900541
TAGACGG79250.012.9469225
TCATTAA927350.012.94625294-95
TATACCG196450.012.694615
CCTAGAC159150.012.3269463
TAGAGCG171600.012.2076875
ATCATTA1038000.012.15651394-95
TATCATT1175600.012.05286394-95
CATTAAA785050.011.93785694-95
TCGCCGT3199950.011.66076994-95
GTATAGG281200.011.3183861
GTCTTAG168200.010.9297451
GTGTATG262250.010.868281
TATACTG252800.010.86082655
GTATAAG249050.010.7004331
Produced by FastQC (version 0.11.5)