FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62999288
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG753600.11962039951943584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2586150.015.23657294-95
CGCCGTA3159750.015.07960394-95
CCGTATC2050550.014.37142594-95
CATTAAA1152250.014.23469894-95
TCATTAA1282850.013.17617994-95
CGTATCA1715950.012.87617294-95
TCGCCGT4024050.011.77346894-95
ATCATTA1407650.011.44770992-93
GTCTAGG204400.010.8769161
CCTAGAC192650.010.8493513
TATACCG171050.010.6919715
TGGTCGC4599850.010.54894190-91
GGTCGCC4578100.010.52021692-93
TATCATT1566100.010.37744592-93
GTCTTAG164200.010.2995351
GTATCAT1598400.010.222836594-95
GTCGCCG4901050.010.04118592-93
CTACCCT257450.010.000614
GTCCTAA210600.09.8799921
TAGAGGT257350.09.8752874
Produced by FastQC (version 0.11.5)